|
KMID : 0942820090080010052
|
|
Journal of Korean Brain Tumor Society
2009 Volume.8 No. 1 p.52 ~ p.62
|
|
|
Purification of Brain Tumor Telomerase and Study on the Telomerase Inhibition
|
|
Hwang Soon-Gu
Lee Young-Woo
|
|
|
Abstract
|
|
|
Objective: Telomerase activity was examined in human brain tumor and compared with their histological diagnosis.
Methods: The 70 brain tumor tissues were surgically obtained from 68 cases at Department of Neurosurgery, Pusan National University Hospital between 1996 and 1999.
Results: The result of telomerase assay of various brain tumor tissues, 6 of 8 glioblastoma multiforme, 7 of 8 anaplastic astrocytoma, 1 of 1 anaplastic oligodendroglioma and 5 of 6 metastatic brain tumor, demonstrated telomerase activity. But epidermoid tumor, cystic teratoma, pituitary adenoma, ependymoma and meningioma did not exhibit high activity. Immunohistochemistry study using anti-CD68 primary antibodies was performed and the proliferative activity of tumor cells were evaluated. By immunohistochemistric study and then, the mean CD68 proliferative cell indexes(PCI) were the followingsglioblastoma(38.4%), anaplastic astrocytoma(18.0%) and anaplastic oligodendroglioma(16.70%). The results
recognized that there was no correlation between telomerase activity and CD68 PCI. But the malignant brain tumors had
higher PCI values than those of benign tumors. The telomerase from brain tumor cells was purified to homogeneity through
one step affinity column chromatography using Ultralink- immorbilized Neutravidin plus beads. The resulting affinity purified
telomerase was active in PCR-amplified telomerase assays. The purified telomerase complex was a molecular weight
of 374 KD and had triple components. The effect of antisense oligonucleotides complementary to the RNA component of
telomerase on telomerase activity in purified enzyme had been studied. Pronounced inhibition of telomerase activity was
observed at the oligonucleotide concentration in the reaction mixture of about 0.4 μM.
Conclusion:
|
|
|
KEYWORD
|
|
|
Human brain tumor, Telomerase, Immunohistochemistry, Proliferative cell index, PCR-amplified assey
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|